FAQ

 

Cell Isolation
Cell Culture Media
Cell Culture Dishes
Antibodies and Proteins

 

 

Cell Isolation

 

Is it possible to isolate NG2 glial progenitors from postnatal brain tissue?

Yes, with the OPC Kit this is possible. OPCs are first generated on MAD-O dishes for 2 days in culture in growth medium (OGM) and then further expanded on PL dishes in OGM. The cellular population obtained consists of NG2+A2B5+ glial progenitors.

 

How can I isolate and culture mature oligodendrocytes from adult brain tissue?

The problem with isolating mature oligodendrocytes from brain tissue of older animals is the mass of myelin debri which would impair the survival of mature oligodendrocytes by activating microglia, myelin lipid release, etc. Another and actually essential technical problem is the handling of mature oligodendrocytes of complex morphology in culture. One should never try to dissociate mature cells once they have been plated into a culture dish. Such treatment would inevitably lead to a disruption of their elaborated process network and cell death. We therefore recommend the use of OPC Kit in a combination with OSDM medium, e.g. first to expand oligodendroglial cells from early postnatal brain tissue in growth medium (OGM), then to plate them into dishes or well-plates for final use, and let them differentiate terminally in our specialized differentiation medium (OSDM).

 

Which mouse or rat strains would be the best to work with the kits?

P.Glia Kits are applicable for any mouse or rat strain. The strains that have so far been tested are C57 Bl, ICR, NMRI (mouse) and Wistar, Sprague Dawley (rat).

 

Is it better to use P2 or P5 rat brains for OPC isolation?

P2 rats would be better, because of lower myelin content, and you could then even skip the density centrifugation step (SDM).

 

Have you any kits or protocols for the isolation and culture of granule cell neurons from mouse or rat cerebellum?

Yes, all you need is our CGN protocoll and specialized media (P.Neural, BSM) and reagents (CDS-1, CDS-2) in order to get millions of granule cell neurons (> 90% pure cell population) within 2 hours work on the bench.

 

 

Cell Culture Media

 

How can I maintain mature oligodendrocytes for longer time in culture?

Our unique oligodendrocyte survival and differentiation medium (OSDM) is specially formulated to support the survival and terminal differentiation of oligodendroglial cells into mature MOG+GalC+MBP+PLP+ oligodendrocytes capable of myelinating axonal processes in vitro.

 

How can I maintain viable and functional cerebellar neurons for longer time in culture?

P.Glia defined neural medium (P.Neural) is best suited for long-term culture and survival (up to 3 weeks) of postnatal cerebellar neurons from mouse or rat under serum-free conditions.

 

 

Cell Culture Dishes

 

 

Antibodies and Proteins